Total RNA of HCC cells was extracted by using a PARISTM Kit (Thermo Fisher Scientific, Waltham, MA, USA) and reverse transcribed by Multiscribe™ Reverse Transcriptase (Thermo Fisher Scientific) agent. Quantitative real-time PCR (qPCR) was performed following the methods described in references.35 (link),36 (link) The level of β-actin mRNA was measured as a loading control. Primers used in qPCR experiments are listed in Table 1 .
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Quantitative RT-PCR Analysis of HCC Cells
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Other organizations : Beijing Radiation Center, Chinese PLA General Hospital
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Variable analysis
independent variables
- None explicitly mentioned
- Level of β-actin mRNA
- β-actin mRNA level as a loading control
- Positive control: None mentioned
- Negative control: None mentioned
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