ATC cells (2 × 103 or 4 × 103 cells) were plated in 96-well low attachment plates (Corning) as previously described [44 (link)]. The number and size of tumor-spheres were measured and photographed by a Zeiss light microscope after 5–14 days.
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Tumor-Sphere Formation Assay
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Corresponding Organization :
Other organizations : National Cancer Institute, National Institutes of Health, Center for Cancer Research
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Variable analysis
independent variables
- Number of ATC cells plated (2 × 10^3 or 4 × 10^3 cells)
- Number of tumor-spheres
- Size of tumor-spheres
- Plating of ATC cells in 96-well low attachment plates (Corning)
- Measurement and photography of tumor-spheres by a Zeiss light microscope after 5–14 days
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