Propofol Exposure on Liver Organoids

Human Lgr5+ cells were isolated from biopsies from livers of two healthy donors as previously described (25 (link)). Use for our studies was approved by the Medical Ethical Committee of our hospital (STEM study). Liver stem cell organoids were passaged to fresh matrigel and cultured in expansion medium (EM) for three days, after which differentiation towards hepatocyte-like cell was started by replacement of EM for differentiation medium (DM). DM was refreshed every 2–3 days and after 5 days, propofol (Fresenius) was added in a concentration of 0, 100, 300 or 900 μM. After 24 hours of incubation with propofol, organoids were harvested for messenger RNA (mRNA) analysis.

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Claesen J.L., Koomen E., Schene I.F., Jans J.J., Mast N., Pikuleva I.A., van der Ham M., de Sain‐van der Velden M.G, & Fuchs S.A. (2020). Misdiagnosis of CTX due to propofol: The interference of total intravenous propofol anaesthesia with bile acid profiling. Journal of Inherited Metabolic Disease, 43(4), 843-851.

Publication 2020

propofol

Biopsies Cells Donors Hepatocyte Human Liver Matrigel Messenger rna Organoids Propofol Stem Stem cell

Corresponding Organization : University Medical Center Utrecht

Other organizations : Case Western Reserve University

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Variable analysis

independent variables

Propofol concentration (0, 100, 300 or 900 μM)

dependent variables

Messenger RNA (mRNA) expression

control variables

Liver stem cell organoid culture conditions (passaged to fresh matrigel, cultured in expansion medium for 3 days, then switched to differentiation medium)
Incubation time with propofol (24 hours)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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