Mannan-Induced Complement Activation Assay
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Corresponding Organization : Mario Negri Institute for Pharmacological Research
Other organizations : University of Milan, Istituto Ortopedico Gaetano Pini
Variable analysis
- Dilution of EDTA-plasma (2.5% in barbital-buffered saline (BBS))
- Incubation time (15 minutes)
- Incubation temperature (37°C)
- Mannan-coated plates (10 µg/mL)
- C3 deposition
- Polyclonal anti-human-C3c antibody (2.4 µg/mL, Dako)
- Alkaline-phosphatase labeled goat anti-rabbit IgG (1 µg/mL, Sigma)
- Optical density (OD) measurement at 405 nm using Infinite M200 spectrofluorimeter (Tecan, CH)
- No positive or negative controls were explicitly mentioned.
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