These were as reported previously30 (link). At sacrifice, the upper portion of the heart and proximal aorta was obtained, embedded in OCT compound, and stored at −70 °C. Serial 10 μm thick cryosections of the aorta, beginning at the aortic root, were collected on poly-D-lysine–coated slides. These sections were stained with Oil Red O and hematoxylin. The images were taken using a Nikon Eclips microscope. Ten sections at 120 μM intervals were counted from each mouse. ImagePro premier software (Media Cybernetics, MD, USA) was used to count the lesion area. This study adhered to the guidelines for experimental atherosclerosis studies as described in the American Heart Association Statement. Circulating leukocyte levels were examined using a HemaTrue analyzer.
Protocol detail
Quantifying Aortic Atherosclerosis in Mice
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Aorta
Aortic root
Atherosclerosis
Cryosections
Heart
Hematoxylin
Leukocyte
Lysine
Microscope
Mouse
Poly
Corresponding Organization :
Other organizations : Charles R. Drew University of Medicine and Science, University of California, Los Angeles, Banaras Hindu University
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Variable analysis
independent variables
- None explicitly mentioned
- Atherosclerotic lesion area
- Circulating leukocyte levels
- Time of sacrifice
- Aortic region (beginning at the aortic root)
- Cryosection thickness (10 μm)
- Staining (Oil Red O and hematoxylin)
- Microscopy (Nikon Eclips microscope)
- Counting method (10 sections at 120 μM intervals, ImagePro premier software)
- None mentioned
- None mentioned
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