The Cu-doped n-CDHA slurry was prepared in a reactor under 40℃ according to a precipitation route described elsewhere.25 Briefly, a certain amount of CuSO4·5H2O (AR, Guang dou Guanghua Sci-Tech co., Ltd) and 10 mole calcium nitrate tetrahydrate (Ca (NO3)2.4H2O, 99.2% Aldrich) was added dropwise to 6 mole diammonium hydrogen phosphate ((NH4)2HPO4, 99.2% Aldrich), and the pH was continuously controlled and adjusted at 9.0±0.1 by adding NH4OH (AR, Chengdou kelong Chemical Reagent Co., Ltd). Precipitate was aged in mother solution for 48 h, washed with NH4OH aqueous solution and alcohol (AR, Sichuan Xilong Chemical co., Ltd). Cu-doped n-CDHA with various Cu/CDHA ratio (W/W, 1.25%, 2.5%, 5%, and 12.5%) were obtained.
The preparation method of Cu-doped n-CDHA/MAC composite with 40 wt% Cu-doped n-CDHA was described in detail in the reference,21 (link) and the scaffold was prepared according to the reference.26 The Cu in the obtained scaffolds were 0.5%, 1%, 2% and 5%, respectively (Figure1 ).![]()
The preparation method of Cu-doped n-CDHA/MAC composite with 40 wt% Cu-doped n-CDHA was described in detail in the reference,21 (link) and the scaffold was prepared according to the reference.26 The Cu in the obtained scaffolds were 0.5%, 1%, 2% and 5%, respectively (
A flow chart about the preparation of scaffold.
