Flow Cytometric Analysis of CD24/CD44 Expression

Cells exposed to indicated concentration of flubendazole for 72 hr, were trypsinized, washed twice with PBS by centrifugation (1,000 rpm, 5 min), and blocked (0.5% BSA/PBS) for 10 min. Collected cells were then incubated with anti-CD24 PE (eBioscience) and anti-CD44 FITC (eBioscience) or isotype controls antibodies according to manufacturer's instructions for 30 min at 4 °C protected from light. Then, cells were suspended in 1 ml PBS and subjected to flow cytometry analysis (Beckman). Side-scatter and forward-scatter profiles were used to eliminate cell doublets [51 (link)]. The statistical results of three independent experiments were presented.