Cell Proliferation and Viability Analysis

A BrdU incorporation assay was performed to analyze the cell proliferation (cycle) of UT7/Epo-S1 cells and CD36⁺ EPCs, followed by flow cytometry analysis, as described previously (57 (link)). The cell viability assay was performed as previously described using a CytoTox-Glo cytotoxicity assay kit (#G9290, Promega) (58 (link)).

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Ning K., Zou W., Xu P., Cheng F., Zhang E.Y., Zhang-Chen A., Kleiboeker S, & Qiu J. (2023). Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors. Science Advances, 9(2), eade0869.

Publication 2023

CytoTox-Glo cytotoxicity assay kit

Assay Brdu Cell proliferation Cell viability Cells Cytotox Epcs Flow cytometry Promega

Corresponding Organization : University of Kansas Medical Center

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Variable analysis

independent variables

Cell type (UT7/Epo-S1 cells and CD36+ EPCs)

dependent variables

Cell proliferation (cycle)
Cell viability

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned.

Annotations

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