This medium was prepared by dissolving 30g of Tryptic Soy Broth powder (BD 211825) in 1L of RO water and autoclaving at 121°C for 15 minutes. Leaf mold medium: Additionally, to investigate the effect on the growth of bacteria cultivated on leaf mold agar media, 10% leaf mold extract was prepared (leaf mold exrract : Milli-Q water = 1:9).
Protocol detail
Leaf Mold Agar Medium Preparation
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Corresponding Organization : Teikyo University
Other organizations : Musashino University
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Variable analysis
independent variables
- Presence or absence of leaf mold extract (10% leaf mold extract in Milli-Q water)
- Growth of bacteria cultivated on leaf mold agar media
- Tryptic Soy Broth powder (BD 211825) concentration (30g in 1L of RO water)
- Autoclaving temperature (121°C)
- Autoclaving duration (15 minutes)
- Positive control: Not specified
- Negative control: Not specified
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