Cultivation of Lyme Disease Spirochete

First passage B. burgdorferi strain B31 clone 5A19 spirochetes, isolated from an ear biopsy of a previously infected mouse, were grown in Barbour-Stoenner-Kelly-H medium supplemented with 6% rabbit serum and antibiotics (rifampicin at 45.4 μg/mL, phosphomycin at 193 μg/ml, and amphotericin at 0.25 μg/ml; Sigma-Aldrich, St. Louis, MO) to late logarithmic phase under microaerophilic conditions. An inoculum containing 1 × 10⁸ spirochetes/ml in RPMI 1640 medium (Invitrogen, USA) was prepared as previously described [11 (link)].

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Martinez A.N., Burmeister A.R., Ramesh G., Doyle-Meyers L., Marriott I, & Philipp M.T. (2017). Aprepitant limits in vivo neuroinflammatory responses in a rhesus model of Lyme neuroborreliosis. Journal of Neuroinflammation, 14, 37.

Publication 2017

rifampicin phosphomycin amphotericin RPMI 1640 medium

Amphotericin Antibiotics Biopsy Clone Mouse Phosphomycin Rabbit Rifampicin Serum Spirochetes Strain

Corresponding Organization :

Other organizations : Tulane University, University of North Carolina at Charlotte

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Variable analysis

independent variables

B. burgdorferi strain B31 clone 5A19 spirochetes

dependent variables

Growth of B. burgdorferi spirochetes

control variables

Barbour-Stoenner-Kelly-H medium supplemented with 6% rabbit serum and antibiotics (rifampicin at 45.4 μg/mL, phosphomycin at 193 μg/ml, and amphotericin at 0.25 μg/ml)
Microaerophilic conditions
RPMI 1640 medium

controls

An inoculum containing 1 × 10^8 spirochetes/ml in RPMI 1640 medium

Annotations

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