The concentration of cytokines, the CXC chemokines KC and Macrophage Inflammatory Protein 2) and cystatin C were determined by enzyme-linked immunosorbent assay (ELISA) using matched antibody pairs (R&D Systems, Inc.) as previously described18 (link). Glypican 1 and 4 were measured using ELISA kits (Cloud Clone, Wuhan China). BUN (Blood Urea Nitrogen), AST (Aspartate Aminotransferase), and Bilirubin were measured by standard clinical methods using kits from Pointe Scientific, Inc (Canton, MI) as previously described15 (link).
Protocol detail
Quantification of Cytokines and Biomarkers
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Antibody
Aspartate aminotransferase
Bilirubin
Blood urea nitrogen
Clone
Cxc chemokines
Cystatin c
Cytokines
Enzyme linked immunosorbent assay
Glypican 1
Macrophage inflammatory protein 2
Corresponding Organization :
Other organizations : Boston Medical Center, Boston University
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Variable analysis
independent variables
- None explicitly mentioned
- Concentration of cytokines (CXC chemokines KC and Macrophage Inflammatory Protein 2)
- Concentration of cystatin C
- Concentration of glypican 1 and 4
- BUN (Blood Urea Nitrogen)
- AST (Aspartate Aminotransferase)
- Bilirubin
- None explicitly mentioned
- Matched antibody pairs (R&D Systems, Inc.) were used for ELISA as previously described18 (link).
- ELISA kits (Cloud Clone, Wuhan China) were used to measure glypican 1 and 4.
- Standard clinical methods using kits from Pointe Scientific, Inc (Canton, MI) were used to measure BUN, AST, and Bilirubin as previously described15 (link).
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