Western Blot Analysis of DNA Damage Response

The details are described in previous report [32 (link)]. For Western blotting, the used antibodies were as following: p21 (OP79; Oncogene Science), p-ATM (Ser1981) (sc-47739; Santa Cruz, CA, USA), p53 (sc-98, Santa Cruz), cleaved caspase-3 (#9661; Cell Signaling, Danvers, MA, USA), phospho-Chk2 (Thr68) (#2197; Cell Signaling), Chk2 (#05-649; EMD Millipore, Temecula, CA, USA). LC3A/B (#12741; Cell Signaling), Chk1 (#2345; Cell Signaling), phospho-Chk1 (Ser345) (#2348; Cell Signaling), phospho-Histone H2A.X (Ser139) (#9718; Cell Signaling), ATM (GTX70103; GeneTex, Irvine, CA, USA), GAPDH (#2118, Cell Signaling), PARP (#9542; Cell Signaling), phospho-p53 (Ser15) (#9284; Cell Signaling). phospho-ATR (Ser428) (#2853; Cell Signaling). PAI-1 (#612024; BD Transduction Laboratories, Lexington, KY, USA).

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Yang S.C., Chen H.Y., Chuang W.L., Wang H.C., Hsieh C.P, & Huang Y.F. (2022). Different Cell Responses to Hinokitiol Treatment Result in Senescence or Apoptosis in Human Osteosarcoma Cell Lines. International Journal of Molecular Sciences, 23(3), 1632.

Publication 2022

p-ATM (Ser1981) cleaved caspase-3 phospho-Chk2 (Thr68) LC3A/B phospho-Chk1 (Ser345) phospho-Histone H2A.X (Ser139) GAPDH phospho-p53 (Ser15) phospho-ATR (Ser428)

Antibodies Caspase 3 Gapdh Histone h2a x Oncogene Pai 1

Corresponding Organization : National Chung Hsing University

Other organizations : Kaohsiung Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of p21, p-ATM (Ser1981), p53, cleaved caspase-3, phospho-Chk2 (Thr68), Chk2, LC3A/B, Chk1, phospho-Chk1 (Ser345), phospho-Histone H2A.X (Ser139), ATM, GAPDH, PARP, phospho-p53 (Ser15), phospho-ATR (Ser428), PAI-1

control variables

None explicitly mentioned

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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