Biomarker Assessment in Fasting Blood

Fasting blood samples were collected from the jugular vein at baseline and monthly. Blood was collected in 0.1 mol/L citrate-containing EDTA. Fresh whole blood was submitted to Antech Diagnostics for CBC with differential and biochemistry measurements (Superchem w/CBC, SA020). Plasma IGF-1 levels were quantified by human IGF-I Quantikine ELISA Kit (R&D Systems, DG100B) and CRP levels by porcine C-reactive protein/CRP DuoSet ELISA (R&D Systems, DY2648). Quantification of plasma N-tyrosine and TAC assay were performed with OxiSelect Nitrotyrosine ELISA Kit (STA-305) and TAC assay kit (STA-360) (both from Cell Biolabs Inc).

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Sukhanov S., Higashi Y., Yoshida T., Danchuk S., Alfortish M., Goodchild T., Scarborough A., Sharp T., Jenkins J.S., Garcia D., Ivey J., Tharp D.L., Schumacher J., Rozenbaum Z., Kolls J.K., Bowles D., Lefer D, & Delafontaine P. (2023). Insulin-like growth factor 1 reduces coronary atherosclerosis in pigs with familial hypercholesterolemia. JCI Insight, 8(4), e165713.

Publication 2023

human IGF-I Quantikine ELISA Kit DuoSet ELISA OxiSelect Nitrotyrosine ELISA Kit TAC assay kit

Assay Blood C reactive protein Cell Citrate Diagnostics Edta Elisa Human Igf i Jugular vein Nitrotyrosine Plasma Porcine Tyrosine

Corresponding Organization : Tulane University

Other organizations : Louisiana State University Health Sciences Center New Orleans, Louisiana State University, Ochsner Medical Center, University of Missouri, Tel Aviv University

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Variable analysis

independent variables

Time of sample collection (baseline, monthly)

dependent variables

CBC with differential
Biochemistry measurements (Superchem w/CBC, SA020)
Plasma IGF-1 levels
Plasma CRP levels
Plasma N-tyrosine levels
Total antioxidant capacity (TAC) assay

control variables

Blood collection in 0.1 mol/L citrate-containing EDTA
Fresh whole blood samples

controls

No positive or negative controls were explicitly mentioned.

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