To analyze the expression levels of HongrES1 in different tissues of R. dorsalis, the whole body, alimentary canal, reproductive organs and salivary gland were dissected from 30 RdFV-free males or virgin females at 5-days post eclosion. The relative expression of HongrES1 in different tissues was detected by RT-qPCR assays. To verify the expression patterns of HongrES1, the total proteins were extracted from various tissues of 30 RdFV-free males or females, and then analyzed by western blot assays. Antibodies against HongrES1 and histone H3 (0.5 μg/μl) served as the primary antibodies, and goat anti-rabbit IgG-peroxidase (0.5 μg/μl) served as the secondary antibody.
We also detected the effects of RdFV or RGDV infection on the expression levels of HongrES1 in the male reproductive system. The reproductive organs were dissected from 30 RdFV-free, RdFV-positive, or RGDV and RdFV co-positive males. The relative expression of HongrES1 was detected by RT-qPCR assays. In the corresponding western blot assay, antibodies against HongrES1, RdFV CP, RGDV P8, and histone H3 (0.5 μg/μl) served as the primary antibodies, and goat anti-rabbit IgG-peroxidase (0.5 μg/μl) served as the secondary antibody. At least three biological replicates were performed.
We also detected the effects of RdFV or RGDV infection on the expression levels of HongrES1 in the male reproductive system. The reproductive organs were dissected from 30 RdFV-free, RdFV-positive, or RGDV and RdFV co-positive males. The relative expression of HongrES1 was detected by RT-qPCR assays. In the corresponding western blot assay, antibodies against HongrES1, RdFV CP, RGDV P8, and histone H3 (0.5 μg/μl) served as the primary antibodies, and goat anti-rabbit IgG-peroxidase (0.5 μg/μl) served as the secondary antibody. At least three biological replicates were performed.
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