To quantify the level of expression of the proteins filaggrin and transglutaminase 3, the Protein Simple Wes capillary-based automated immunoassays platform was used (24 (link)). RHE were treated as previously described for morphology and permeability experiments (see above Materials and Methods, Reconstructed human epidermis treatment). For these experiments, in addition to sterile water and mDixon + SQ 2%, M. restricta 24 h supernatant B was used and the SQOOH concentration was adapted accordingly to 193 ng/ml in mDixon + SQ 2%, MDA at 60 ng/ml in mDixon + SQ 2%, and SQOOH at 193 ng/ml + MDA at 60 ng/ml in mDixon + SQ 2%. Each solution was applied in triplicate. Samples were lysed using a specific buffer with Precellys® lysing kits (Bertin Technologies) designed for soft tissue homogenizing and dosed by bicinchoninic acid (BCA) assay. Diluted protein lysate was mixed with fluorescent master mix and heated at 37°C for 10 min. The plate was loaded into the instrument (WES, Protein Simple) and protein was drawn into individual capillaries on a 25-capillary cassette (12-230 kDa or 66-440 kDa) provided by the manufacturer (SM-W001). Proteins of interest were immunodetected with primary antibodies targeting FLG or TGM3 and peroxidase-conjugated secondary antibodies (Goat anti-Mouse or Rabbit IgG-HRP). Signals were normalized to cofilin immunodetection.