Todd Hewitt broth (THB, Sigma-Aldrich) was prepared and supplemented with 10 µM menadione and 4 mg/mL hemin (ThermoFisher) and mixed 1:1 with Roswell Park Memorial Institute media (RPMI). Referred to as sTHB from herein. A similar media has been described elsewhere and has been shown to enable the growth of both fungi and bacteria (Montelongo-Jauregui et al., 2016 (link)).
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