Resting-State fMRI Brain Imaging Protocol

All MRI and assessment procedures were identical across assessment occasions^{64 (link)}. Scanning was performed using a 3.0T MRI scanner (Siemens Magnetom, Trio Tim, Erlangen, Germany) equipped with a 12-channel array coil located at the Wolfson Brain Imaging Centre, Addenbrooke’s Hospital, Cambridge, UK. T2*-weighted echo-planar images were acquired under eyes-closed resting-state conditions. Participants were instructed to close their eyes and let the minds wander without going to sleep. Subsequent participant debriefing ensured that no participants fell asleep during the scan. Imaging parameters were: 3 × 3 × 3.75 mm voxel size, with a time-to-repetition (TR) of 2000 ms, time-to-echo (TE) of 30 ms, flip angle of 781 in 64 × 64 matrix size, and 240 mm field of view (FOV). A total of 300 volumes comprising 32 slices each were obtained. In addition, high-resolution anatomical T1 images were acquired using a three-dimensional magnetic-prepared rapid gradient echo (MPPRAGE) sequence. In all, 176 contiguous sagittal slices of 1.0 mm thickness using a TR of 2300 ms, TE of 2.98 ms, flip angle of 91, and a FOV of 256 mm in 240 × 256 matrix were acquired with a voxel size of 1.0 mm³. One participant was excluded due to excessive movement, resulting in a final sample of N = 20 subjects.