High-fat Diet and Phloretin Effects

Ten-week-old male specific pathogen-free C57BL/6J wild-type mice were purchased from Damul Science (Laboratory Animal Supplier Registration No.10, Daejeon, South Korea) and fed with a 45% kcal high-fat diet D12451 (45 kcal% of energy from fat, 20 kcal% of energy from protein, and 35 kcal% of energy from carbohydrate) (Lot: 22090804, Research Diets, New Brunswick, NJ, USA) or standard diet (SD) for 12 weeks under the free-feeding condition. They were individually housed and kept at a temperature of 24 °C on a 12 h light/dark cycle. Phloretin (Lot: 20201216, Yibin Ereal Chemical, Yibin, China) and 3-OH phloretin solubilized with 0.5% DMSO were administered orally at 10 mg/kg three times a week during 12 weeks of HFD feeding, as described previously [22 (link)]. The purity of phloretin and 3-OH phloretin was 99% and 98%, respectively. All animal experiments were reviewed and approved by the Chonnam National University Institutional Animal Care and Use Committee (Ethical approval code: CNU IACUC-YB-2021-151).