Scotopic Electroretinography in Dark-Adapted Mice

Animals were dark-adapted overnight before recording scotopic responses under anesthesia and under dim red light exactly as described previously using a UTAS-E2000 visual electrodiagnostic system (LKC Technologies, Gaithersburg, MD)^{26 (link)}. Stimuli were presented in order of increasing intensity as a series of white flashes of 1.5 cd-s/m² attenuated to yield intensities from −1.8 to 0.2 log cd-s/m². For each flash intensity, three to six recordings were averaged. For all recordings, a-wave amplitudes were measured from the baseline to the trough of the a-wave, and b-wave amplitudes were measured from the trough of the a-wave to the peak of the b-wave.