Mice were housed (n = 1 or 2) in metabolic cages, and 48 hr collections (1–2 mice per urine pool) were made under mineral oil into vessels containing crystal thymol as a preservative. Urinary oxalate (mg/dl) and creatinine (mg/dl) concentrations were determined in acidified (HCl) samples collected from all mice over a 48 hr period by Litholink Corp (Chicago, IL). Fecal pellets were collected at the end of the urine collections. Fecal pellets were acidified using 2M HCl, vortexed for 20 min, and then centrifuged at 21,000 g at room temperature, and supernatant fecal water collected using described methods (Jiang et al., 2011 (link)). Fecal water oxalate was measured using an oxalate calorimetric assay (Abcam, ab196990, Cambridge, UK) per the manufacturer’s instructions. Technical replicates are repeated measurements of the same sample that represent independent measures of the random noise associated with protocols or equipment. Fecal oxalate and qPCR were measured in duplicates.
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