Airway Hyperresponsiveness Measurement in Mice

The mice were intranasally challenged via rmIL-33 (BioLegend, San Diego, Calif) or Alternaria alternata extracts (Greer Laboratories, Lenoir, North Carolina), as shown in the experimental schemes. For CD200R engagement, mouse CD200-Fc (3355-CD-050; R&D Systems), human CD200-Fc (2724-CD-050; R&D Systems) and the corresponding IgG1 isotypes (110-HG-100; R&D Systems) were used. Lung function was measured by direct measurement of lung resistance and dynamic compliance in anesthetized tracheostomized mice, in which mice were mechanically ventilated via the FinePointe RC system (Buxco Research Systems, Wilmington, NC), and sequentially challenged with aerosolized increasing doses of methacholine^{38 (link)}. Maximum lung resistance and minimum compliance values were recorded during a 3-min period after each methacholine challenge. AHR data were analyzed by repeated measurements of a general linear model.

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Shafiei-Jahani P., Helou D.G., Hurrell B.P., Howard E., Quach C., Painter J.D., Galle-Treger L., Li M., Loh Y.H, & Akbari O. (2021). CD200–CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma. Nature Communications, 12, 2526.

Publication 2021

rmIL-33 Alternaria alternata extracts

Alternaria alternata Cd200 Human Igg1 Isotypes Lung Lung function Methacholine Mice Mouse cd200 fc

Corresponding Organization :

Other organizations : University of Southern California

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Variable analysis

independent variables

RmIL-33 (BioLegend, San Diego, Calif)
Alternaria alternata extracts (Greer Laboratories, Lenoir, North Carolina)
Mouse CD200-Fc (3355-CD-050; R&D Systems)
Human CD200-Fc (2724-CD-050; R&D Systems)
Corresponding IgG1 isotypes (110-HG-100; R&D Systems)

dependent variables

Lung function measured by direct measurement of lung resistance and dynamic compliance in anesthetized tracheostomized mice
Maximum lung resistance and minimum compliance values recorded during a 3-min period after each methacholine challenge

control variables

Mice were mechanically ventilated via the FinePointe RC system (Buxco Research Systems, Wilmington, NC)
Mice were sequentially challenged with aerosolized increasing doses of methacholine

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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