Metabolomics Assay of P. ternata Leaves

The samples used for the metabolomics assay were consistent with the transcriptome, both being P. ternata leaves. Using an ACQUITY UPLC ® HSS T3 (2.1 150 mm, 1.8 m) (Waters, Milford, MA, USA) chromatographic column, the Thermo Vanquish (Thermo Fisher Scientific, USA) ultra-high performance liquid phase system operates at a flow rate of 0.25 mL/min, a column temperature of 40°C, and an injection volume of 2 µL (Liu et al., 2020b (link); Fu et al., 2021 (link)).

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Wang D., Sarsaiya S., Qian X., Jin L., Shu F., Zhang C, & Chen J. (2023). Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data. Frontiers in Plant Science, 14, 1227507.

Publication 2023

ACQUITY UPLC ® HSS T3 Thermo Vanquish

Assay Chromatographic Transcriptome

Corresponding Organization : Zunyi Medical University

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Variable analysis

independent variables

Flow rate (0.25 mL/min)
Column temperature (40°C)
Injection volume (2 µL)

dependent variables

Metabolomics data

control variables

ACQUITY UPLC ® HSS T3 (2.1 150 mm, 1.8 m) chromatographic column
Thermo Vanquish ultra-high performance liquid phase system
Sample material (P. ternata leaves)

controls

No positive or negative controls were explicitly mentioned.

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