Chinese hamster ovary (CHO) E77.4 cells79 (link) from ATCC were cultured in RPMI-1640 medium (Quality Biological 112-025-101) containing 10% heat-inactivated fetal bovine serum (Gibco™ 10082147), 2mM L-Glutamine (Quality Biological 118-084-721), and 100 U/mL Penicillin with 100 µg/mL Streptomycin (Quality Biological 120-095-721). Passages were performed using 0.05% Trypsin-0.1% EDTA (Quality Biological 118-087-721).
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