Immunofluorescence Analysis of AGR2, WFDC2, and TSC22D3

Immunofluorescence studies for the detection of AGR2 (1 in 100 dilution) (Abcam, Cat No: ab227584), WFDC2 (1 in 250 dilution) (Abcam, Cat No: ab200828) and TSC22D3 (1 in 50 dilution) (Abcam, Cat No: ab197987) were performed as described previously [24 (link),33 (link),34 (link)]. Epithelial expression of AGR2, WFDC2 and TSC22D3 positive cells was confirmed using cytokeratin (1 in 500) (Biocare, Denmark) and appropriate Alexa fluorophor conjugated secondary antibodies (Thermo Fisher Scientific, Austin, TX, USA). Localization of AGR2 protein expression to the endoplasmic reticulum (ER) was confirmed using the ER marker GRP94 (1 in 1000 dilution) (Abcam, Cat No: ab210960).

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Alvarez X., Sestak K., Byrareddy S.N, & Mohan M. (2020). Long Term Delta-9-tetrahydrocannabinol Administration Inhibits Proinflammatory Responses in Minor Salivary Glands of Chronically Simian Immunodeficieny Virus Infected Rhesus Macaques. Viruses, 12(7), 713.

Publication 2020

ab227584 ab200828 ab210960

Agr2 protein Antibodies Austin Cells Cytokeratin Dilution Endoplasmic reticulum Grp94 Immunofluorescence Tsc22d3 Wfdc2

Corresponding Organization : University of Nebraska Medical Center

Other organizations : Transtria (United States), Tulane University

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Variable analysis

independent variables

Dilution of AGR2 (1 in 100)
Dilution of WFDC2 (1 in 250)
Dilution of TSC22D3 (1 in 50)

dependent variables

Epithelial expression of AGR2, WFDC2 and TSC22D3 positive cells
Localization of AGR2 protein expression to the endoplasmic reticulum (ER)

control variables

Cytokeratin (1 in 500) to confirm epithelial expression
GRP94 (1 in 1000 dilution) to confirm localization of AGR2 to the ER

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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