Quantification of Paeonol by HPLC

The paeonol standard was precisely weighed at 5.0 mg, placed in 10 mL volumetric flasks, dissolved with methanol, and shaken well to obtain a paeonol standard solution with a concentration of 0.5 mg/mL. A series of paeonol standard solutions (5, 10, 15, 20, 25 µg/mL) were prepared by removing 0.1, 0.2, 0.3, 0.4, and 0.5 mL of the paeonol standard solution with a pipette and adjusting the volume precisely to 10 mL with a methanol solution. The standard curve was drawn with the peak area y as the vertical coordinate and the paeonol concentration x as the horizontal coordinate. The regression equation of paeonol was y = 84,218x – 10,221, R² = 0.9996.