Isolation and Characterization of Potato-Associated Pseudomonas Strains

The four Pseudomonas strains used for this study were isolated in 2015 from field-grown potatoes as described in [9 (link),25 (link)], two from the roots (Pseudomonas donghuensis R32, P. chlororaphis R47) and two from the shoots (Pseudomonas sp. S04, Pseudomonas sp. S35). We selected these strains because they differed in their protective activity against P. infestans and in their ability to emit VOCs on laboratory media. The strains were kept in 25% (v/v) glycerol for long-term storage and passaged weekly on 1.5% agar (w/v; Erne, Switzerland) LB medium (20 g/L; Applichem, Schaffhausen, Switzerland) or ABG medium [26 (link)] for routine use.

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Gfeller A., Fuchsmann P., De Vrieze M., Gindro K, & Weisskopf L. (2022). Bacterial Volatiles Known to Inhibit Phytophthora infestans Are Emitted on Potato Leaves by Pseudomonas Strains. Microorganisms, 10(8), 1510.

Publication 2022

LB medium

Agar Emit Glycerol Potatoes Pseudomonas Pseudomonas donghuensis Roots Strains

Corresponding Organization : University of Fribourg

Other organizations : Agroscope

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Variable analysis

independent variables

Pseudomonas strains used for the study: Pseudomonas donghuensis R32, P. chlororaphis R47, Pseudomonas sp. S04, and Pseudomonas sp. S35

dependent variables

Protective activity against P. infestans
Ability to emit VOCs on laboratory media

control variables

Storage conditions: 25% (v/v) glycerol for long-term storage
Routine culture conditions: Weekly passage on 1.5% agar (w/v) LB medium (20 g/L) or ABG medium

controls

No positive or negative controls were explicitly mentioned in the provided information.

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