Carotenoid Analysis by UHPLC-DAD-MS
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Corresponding Organization : The Ohio State University
Protocol cited in 1 other protocol
Variable analysis
- Dried extracts were redissolved in 1 mL MtBE–MeOH (1:1)
- Carotenoids were separated and quantified
- Filtered with a 0.22 μm nylon filter (CellTreat)
- Carotenoids were separated on a C18 Acquity BEH column (Waters Corp.) 2.1 × 150 mm, 1.7 μm particle size, maintained at 55°C
- An isocratic flow using 42% solvent A [80% MeOH, 20% water, and 2% (w/v) aqueous ammonium acetate] and 58% solvent B [78% MtBE, 20% MeOH, and 2% (w/v) aqueous ammonium acetate] at a flow rate of 0.45 mL/min was used, and each run lasted 4.2 min
- Quantification was achieved by six-point external calibration curves
- Carotenoid identities were confirmed by authentic standards, spectral characteristics, and tandem MS using a 6495 triple quadrupole MS (Agilent) with an atmospheric pressure chemical ionization source operated in positive mode
- Authentic standards for carotenoid identification
- Not explicitly mentioned
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