Western Blot Analysis of IL-6 Signaling Pathway

Western blotting was performed in accordance with our previously described procedures [18 (link)]. The following primary antibodies were diluted according to the manufacturers’ recommendations: anti-human bile acid receptor NR1H4 (cat. no. ab187735; Abcam), anti-IL-6 (cat. no. ab9324; Abcam), anti-CD130 (gp130) (cat. no. ab283685; Abcam), anti-phosphorylated (p)-Jak2 (Tyr1007/1008) (cat. no. 3771s; Cell Signaling Technology, Inc.), anti-Jak2 (cat. no. 3230s; Cell Signaling Technology, Inc.), anti-p-STAT3 (Tyr705) (cat. no. 9145s; Cell Signaling Technology, Inc.), anti-STAT3 (cat. no. 12640s; Cell Signaling Technology, Inc.), anti-IL-6Rα/CD126 (cat. no. 18935s; Cell Signaling Technology, Inc.), anti-YAP (cat. no. 12395s; Cell Signaling Technology, Inc.), anti-HES1 (cat. no. 11988s; Cell Signaling Technology, Inc.), anti-Cleaved Notch1 (cat. no. 4147s; Cell Signaling Technology, Inc.), anti-GAPDH (cat. no. 92310sf; Cell Signaling Technology, Inc.), and anti-p-YAP1 (Tyr357) (cat. no. Y0771; Sigma-Aldrich, Inc., USA).