Western Blot Antibody Protocols

The following antibodies were used for Western Blot analysis: polyclonal anti-caspase-3 antibody (#9662) and monoclonal anti-RIPK1 XP antibody (#3493) from Cell Signaling; polyclonal anti-actin antibody (A2103; Sigma-Aldrich, Germany), polyclonal anti-mCherry antibody (ab183628; Abcam), monoclonal anti-caspase-10 antibody (M059–3; MBL International), monoclonal anti-FADD antibody (clone 1C4), monoclonal anti-caspase-8 antibody (clone C15) and monoclonal c-FLIP antibody (clone NF6). Horseradish peroxidase-conjugated goat anti-mouse IgG1,-2a,-2b, goat anti-rabbit and rabbit anti-goat were from Santa Cruz (California, USA). All chemicals were of analytical grade and purchased from Merck (Darmstadt, Germany) or Sigma-Aldrich (Germany). The C15, NF6 and 1C4 antibodies were kindly provided by Prof. P. H. Krammer (DKFZ, Heidelberg). Recombinant LZ-CD95L was produced as described [25 (link)].

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Ivanisenko N.V., Buchbinder J.H., Espe J., Richter M., Bollmann M., Hillert L.K., Ivanisenko V.A, & Lavrik I.N. (2019). Delineating the role of c-FLIP/NEMO interaction in the CD95 network via rational design of molecular probes. BMC Genomics, 20(Suppl 3), 293.

Publication 2019

polyclonal anti-caspase-3 antibody polyclonal anti-actin antibody polyclonal anti-mCherry antibody (ab183628;

Actin Anti antibody Antibodies Caspase 10 Caspase 3 Caspase 8 Cd95l Clone Fadd Goat Horseradish peroxidase Igg1 Monoclonal antibody Mouse Rabbit Ripk1 Western blot analysis

Corresponding Organization :

Other organizations : Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Otto-von-Guericke University Magdeburg, Novosibirsk State University

Top 5 similar protocols

Variable analysis

independent variables

Polyclonal anti-caspase-3 antibody (#9662) from Cell Signaling
Monoclonal anti-RIPK1 XP antibody (#3493) from Cell Signaling
Polyclonal anti-actin antibody (A2103; Sigma-Aldrich, Germany)
Polyclonal anti-mCherry antibody (ab183628; Abcam)
Monoclonal anti-caspase-10 antibody (M059–3; MBL International)
Monoclonal anti-FADD antibody (clone 1C4)
Monoclonal anti-caspase-8 antibody (clone C15)
Monoclonal c-FLIP antibody (clone NF6)
Horseradish peroxidase-conjugated goat anti-mouse IgG1,-2a,-2b, goat anti-rabbit and rabbit anti-goat from Santa Cruz (California, USA)
Recombinant LZ-CD95L

dependent variables

Not explicitly mentioned

control variables

All chemicals were of analytical grade and purchased from Merck (Darmstadt, Germany) or Sigma-Aldrich (Germany)

controls

Positive control: Recombinant LZ-CD95L
Negative controls: Not explicitly mentioned

Annotations

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