Spa Typing of Livestock-Associated S. Aureus

The spa typing was performed using the Ridom StaphType 2.2.1 software and the Ridom SpaServer (http://spa.ridom.de/index.shtml) (European Union Reference Laboratory for Antimicrobial Resistance 2009 ). In previous work, we have assigned spa types to putative CC398 or CC9 using studies that report both spa types and multi-locus sequence types (MLST) for livestock-associated S. aureus isolates (Hasman et al. 2010 (link); Lozano et al. 2012 (link); Price et al. 2012 (link)). However, a recent study of hogs and hog veterinarians in the United States reported that some spa types typically associated with CC9 (i.e., t337 and t3446) clustered with CC398 when MLST was performed (Sun 2016 ). Thus, we assigned S. aureus spa types as “putative CC398 or CC9” in this study, rather than to each CC uniquely.

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Nadimpalli M.L., Stewart J.R., Pierce E., Pisanic N., Love D.C., Hall D., Larsen J., Carroll K.C., Tekle T., Perl T.M, & Heaney C.D. (2018). Face Mask Use and Persistence of Livestock-associated Staphylococcus aureus Nasal Carriage among Industrial Hog Operation Workers and Household Contacts, USA. Environmental Health Perspectives, 126(12), 127005.

Publication 2018

SpaServer

Antimicrobial Livestock S aureus Veterinarians

Corresponding Organization : Johns Hopkins University

Other organizations : University of North Carolina at Chapel Hill, Empowerment Program, Statens Serum Institut, Johns Hopkins Medicine, Johns Hopkins Hospital, The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

Spa typing performed using the Ridom StaphType 2.2.1 software and the Ridom SpaServer

dependent variables

Assignment of spa types to putative CC398 or CC9 using studies that report both spa types and multi-locus sequence types (MLST) for livestock-associated S. aureus isolates

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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