Transmission Electron Microscopy Analysis of Engineered Nanomaterial Cellular Uptake

ENM cellular uptake was confirmed by TEM imaging. TT1 cells, the primary focus of this study were exposed to ENM were fixed, embedded, sectioned and imaged as previously described [27 (link)]. The analysis was performed with a FEI Titan3 Themis G2 operating at 300 kV fitted with 4 EDX silicon drift detectors, and a Gatan One-View CCD. EDX spectroscopy and mapping to identify potential heavy metal contamination was undertaken using Bruker Esprit v1.9 software.

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Burgum M.J., Clift M.J., Evans S.J., Hondow N., Tarat A., Jenkins G.J, & Doak S.H. (2021). Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro. Journal of Nanobiotechnology, 19, 24.

Publication 2021

Titan3 Themis G2 One-View CCD

Cells Esprit Heavy metal Silicon Spectroscopy

Corresponding Organization :

Other organizations : Swansea University, University of Leeds, Olympus (United Kingdom)

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Variable analysis

independent variables

Exposure of TT1 cells to ENM

dependent variables

Cellular uptake of ENM confirmed by TEM imaging

control variables

TT1 cells were the primary focus of this study
Cells were fixed, embedded, sectioned and imaged as previously described [27]
EDX spectroscopy and mapping was undertaken to identify potential heavy metal contamination

controls

No positive or negative controls were explicitly mentioned in the provided information.

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