Western Blotting of Cytoskeletal Proteins

Cells were collected in lysis buffer as described (Zaoui et al., 2008 (link)). Western blotting was performed using antibodies directed against mDia1, IQGAP1, HAX1 (BD Biosciences, Le Pont de Claix, France), Dia2c (Santa Cruz Biotechnology, Dallas, TX), mDia2/DRF3 (a kind gift from H. N. Higgs, Dartmouth Medical School, Hanover, NH), α-tubulin, ELKS (Sigma-Aldrich, Lyon, France), and GFP (Roche, Meylan, France).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Daou P., Hasan S., Breitsprecher D., Baudelet E., Camoin L., Audebert S., Goode B.L, & Badache A. (2014). Essential and nonredundant roles for Diaphanous formins in cortical microtubule capture and directed cell migration. Molecular Biology of the Cell, 25(5), 658-668.

Publication 2014

IQGAP1 HAX1 α-tubulin

Antibodies Buffer Cells Drf3 Elks α tubulin

Corresponding Organization : Institut Paoli-Calmettes

Other organizations : Brandeis University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Lysis buffer used to collect cells

dependent variables

Protein levels of mDia1, IQGAP1, HAX1, Dia2c, mDia2/DRF3, α-tubulin, ELKS, and GFP as measured by Western blotting

control variables

Not explicitly mentioned

controls

Positive control: None specified
Negative control: None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!