Genotyping of Sentinel SNPs and AIMs

A sentinel SNP for each region was selected for genotyping from previously reported GWAS (1 (link)–17 (link)). Two SNPs were selected for KCNQ1 and CDC123, where two distinct sets of variants have been described. In addition, 45 ancestry-informative markers (24 (link)) were genotyped for estimation of the individual proportion of European heritage (25 (link)). Genotyping was conducted by the SNPplex method (Life Technologies, Carlsbad, CA) or the BeadXpress system (Illumina, San Diego, CA) according to the manufacturer’s instructions. Results for 18 SNPs were reported previously (20 (link),26 (link)–29 (link)). They are included here for a more complete characterization of the effects of established T2DM loci.

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Hanson R.L., Rong R., Kobes S., Muller Y.L., Weil E.J., Curtis J.M., Nelson R.G, & Baier L.J. (2015). Role of Established Type 2 Diabetes–Susceptibility Genetic Variants in a High Prevalence American Indian Population. Diabetes, 64(7), 2646-2657.

Publication 2015

BeadXpress system

European Gwas Snps

Corresponding Organization :

Other organizations : National Institute of Diabetes and Digestive and Kidney Diseases

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Variable analysis

independent variables

Sentinel SNPs for each region from previously reported GWAS
Two SNPs selected for KCNQ1 and CDC123
45 ancestry-informative markers

dependent variables

Individual proportion of European heritage

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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