Two DLBCL-NOS cell lines (GR, HBL1) and six HGBCL-DH cell lines (MZ, TMD8, OCI-LY10, OCI-LY9, RC, and MCA) were kind gifts from MD Anderson Cancer Center Laboratory (Drs. Pham and Ford; refs. 34 (link)–36 (link)). We performed targeted next-generation sequencing, IHC, and FISH to determine the TP53, MYC, and BCL2 alteration status in all DLBCL cell lines. Cells were cultured in RPMI1640 (CORNING) supplemented with 10% to 15% FBS (Gibco, Life Technologies) with 100 U/mL penicillin and 100 μg/mL streptomycin at 37°C in a humidified atmosphere with 5% CO2. All cultured cell lines were routinely tested for Mycoplasma spp. using a MycoSEQ Mycoplasma Detection kit (Invitrogen). Stocks of authenticated cell lines were cryopreserved, and all cell lines used here were obtained from these authenticated stocks and passaged for <6 months in culture for the performed experiments. INCB057643 and DS3032b were supported separately by Incyte and Daiichi Sankyo under approved material transfer agreement. Venetoclax (ABT-199) was purchased from Selleckchem.
Protocol detail
Characterization of DLBCL and HGBCL Cell Lines
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Abt 199
Atmosphere
Bcl2
Cancer
Cell lines
Cells
Fish
Gifts
Incb057643
Mycoplasma
Penicillin
Streptomycin
Tp53
Venetoclax
Corresponding Organization : Incyte (United States)
Other organizations : Pharmacyclics (United States), University Hospital of Basel, University of Verona, NewYork–Presbyterian Hospital, Columbia University Irving Medical Center, Aalborg University Hospital, Mayo Clinic, Cornell University, Cleveland Clinic, Guangzhou Medical University, Asan Medical Center, San Raffaele University of Rome, Odense University Hospital, Lutheran Hospital, The University of Texas MD Anderson Cancer Center, Radboud University Medical Center, Radboud University Nijmegen, Marqués de Valdecilla University Hospital, Northwestern University, Baylor College of Medicine
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- INCB057643
- DS3032b
- Venetoclax (ABT-199)
- TP53 alteration status
- MYC alteration status
- BCL2 alteration status
- Cell culture conditions (RPMI1640 medium, 10-15% FBS, penicillin, streptomycin, 37°C, 5% CO2)
- Mycoplasma testing using MycoSEQ Mycoplasma Detection kit
- Cell line authentication and passage number (<6 months)
- Not specified
- Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!