Kunming (KM) mice are outbreeding mice, they came from swiss mice. SPF (specific pathogen free) KM mice (six weeks old, male) were obtained from the Laboratory Animal Center, Chongqing Medical University, and raised at a temperature of 22 ± 4 °C and a humidity of 50 ± 20%. The mice were randomly divided into five groups (n = 10): normal group, control group, low-dose PRLT administrated by gavage group (PRLT-L group, 100 mg/kg), high-dose PRLT administrated by gavage group (PRLT-H group, 200 mg/kg), and ranitidine administrated by gavage group (50 mg/kg). After a one-week acclimation, normal food and drink were allowed for the mice in the normal group and control group. PRLT solutions at 100 mg/kg (0.2 mL) and 200 mg/kg (0.2 mL) were administrated by gavage in the PRLT-L group and PRLT-H group, respectively. Ranitidine solution at 50 mg/kg (0.2 mL) was administrated by gavage in the ranitidine group. The doses were given for two weeks. At Day 14, the mice were not allowed to eat but had free access to water. After fasting for 24 h, except for the mice in the normal group, other mice were administrated with inducer (60% ethanol and 40% 150 mmol/HCl) by gavage. After 30 min, all mice were dissected to collect gastric juices and gastric tissues. The eyeballs were extracted using a tweezer [22 (link)]. The gastric area was photographed, and the injury area was measured via ImageJ, based on which the inhibition rate of gastric injury by PRLT was calculated. This study was approved by the Animal Ethics Committee of Chongqing University of Education (Chongqing, China), Laboratory animal using license No. SYXK (Yu) 2018-0003.
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