Plasma Metabolite Quantification and Cortisol Analysis

Plasma metabolites (triglycerides and total protein) were quantified by a photometric method using Cobas™ C111 Chemistry Analyzer (Roche Diagnostics International Ltd. Rotkruez, Switzerland). The system was calibrated using tetramethylammonium chloride (C.f.a.s Calibrator; Roche diagnostics GmbH, Mannheim Germany). Analytic controls (PreciControl ClinChem Multi 1 and 2; Roche diagnostics GmbH, Mannheim Germany) consisting of lyophilized human sera were used for quality control. Triglycerides were quantified using the TRIGL kit (Roche diagnostics GmbH, Mannheim, Germany) and total protein concentration was measured using the TP2 Kit (Roche diagnostics GmbH) according to the manufacturer's protocols. Steroid extraction for cortisol analysis was performed according to Aizen et al. (42 (link)) and cortisol concentrations were measured using a cortisol-specific ELISA according to the protocol published by Yeh et al. (43 (link)).

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Segev-Hadar A., Alupo G., Tal K., Nitzan T, & Biran J. (2020). Identification and Characterization of a Non-muscular Myostatin in the Nile Tilapia. Frontiers in Endocrinology, 11, 94.

Publication 2020

Cobas™ C111 Chemistry Analyzer C.f.a.s Calibrator PreciControl ClinChem Multi 1 and 2 TRIGL kit TP2 Kit

Cortisol Diagnostics Elisa Human Photometric method Plasma Protein Sera Steroid Tetramethylammonium chloride Triglycerides

Corresponding Organization : Agricultural Research Organization

Other organizations : Hebrew University of Jerusalem

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Variable analysis

independent variables

Plasma metabolites (triglycerides and total protein) were quantified

dependent variables

Triglycerides
Total protein concentration
Cortisol concentrations

control variables

The system was calibrated using tetramethylammonium chloride (C.f.a.s Calibrator; Roche diagnostics GmbH, Mannheim Germany)
Analytic controls (PreciControl ClinChem Multi 1 and 2; Roche diagnostics GmbH, Mannheim Germany) consisting of lyophilized human sera were used for quality control

positive controls

PreciControl ClinChem Multi 1 and 2 (lyophilized human sera)

negative controls

Tetramethylammonium chloride (C.f.a.s Calibrator)

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