1mg of rhein was taken in 10mL of volumetric flask and was dissolved in 5 mL of acetonitrile and made upto 10ml with acetonitrile to give primary stock solution to produce concentration 100µg/ml. Dilutions were prepared out of primary stock solution in the concentration range 2 to 10µg/mL by pipetting 0.2, 0.4, 0.6, 0.8, 1ml and diluting each of them to 10ml with diluent. The absorbance was measured for each by UV spectrophotometer at λmax 430nm. The graph was plotted for absorbance vs concentration.
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Rhein Quantification by UV Spectroscopy
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Variable analysis
independent variables
- Concentration of rhein in the dilutions, ranging from 2 to 10 µg/mL
- Absorbance measured by UV spectrophotometer at λmax 430nm
- Volume of primary stock solution (100 µg/mL) used for dilutions (0.2, 0.4, 0.6, 0.8, 1 mL)
- Total volume of dilutions made up to 10 mL with diluent
- Wavelength of UV spectrophotometer (430 nm)
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