Gal-9 and CD44 Colocalization in NK Cells

Surface-stained mouse splenocytes (1 x 10⁶) were fixed in 4% paraformaldehyde. An Amnis ImageStream Mark II (EMD Millipore, ON, Canada) was used to collect at least 10,000 images for each sample as we have previously described (31 (link)). Data were analyzed using the IDEAS analysis software package to select single-cell images that were in focus. Next, we performed gating for NK cells (CD49b⁺) followed by gating for cells that co-express Gal-9 and CD44. Colocalization of Gal-9 and CD44 was determined using the IDEAS colocalization wizard.

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Rahmati A., Bigam S, & Elahi S. (2023). Galectin-9 promotes natural killer cells activity via interaction with CD44. Frontiers in Immunology, 14, 1131379.

Publication 2023

Amnis ImageStream Mark II

Cd44 Cells Gal 9 Mark ii Mouse Nk cells Paraformaldehyde

Corresponding Organization : University of Alberta

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Variable analysis

independent variables

Surface-stained mouse splenocytes (1 x 10^6)

dependent variables

Colocalization of Gal-9 and CD44

control variables

Fixed in 4% paraformaldehyde
Gating for NK cells (CD49b+)
Gating for cells that co-express Gal-9 and CD44

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