Telomere Length Measurement by STELA

STELA was conducted as described previously, using genomic DNA (250 pg/reaction) for telomeres at 12q (12qSTELA primer), 17p (17p6 primer) and XpYp (XpYpE2 primer), and 500 pg/reaction for the ciHHV-6-associated telomere (DR1R primer)25 (link)^,34 (link)56 (link). Individual amplicons, detected by phosphor-image analysis, were sized using the Imagequant software (Typhoon 9400, GE Healthcare) with known size markers (GeneRuler 1 kb and GeneRuler High Range DNA ladder, Fermentas), and the length of the flanking sequence was subtracted. The telomere lengths were presented as scatter plots with median and interquartile ranges. Telomere lengths in the blood and PEL samples were compared using the non-parametric Kruskal-Wallis test (GraphPad Prism; GraphPad Software Inc., CA, USA).

Free full text: Click here

Zhang E., Cotton V.E., Hidalgo-Bravo A., Huang Y., J. Bell A., F. Jarrett R., Wilkie G.S., Davison A.J., P. Nacheva E., Siebert R., Majid A., Kelpanides I., Jayne S., Dyer M.J, & Royle N.J. (2016). HHV-8-unrelated primary effusion-like lymphoma associated with clonal loss of inherited chromosomally-integrated human herpesvirus-6A from the telomere of chromosome 19q. Scientific Reports, 6, 22730.

Publication 2016

Typhoon 9400

Blood Cihhv 6 Genomic Phosphor Primer Prism Telomere Typhoon

Corresponding Organization :

Other organizations : University of Leicester, MRC University of Glasgow Centre for Virus Research, Royal Free London NHS Foundation Trust, University Hospital Schleswig-Holstein, University of Lübeck, Kiel University

Top 5 similar protocols

Variable analysis

independent variables

Genomic DNA (250 pg/reaction) for telomeres at 12q (12qSTELA primer), 17p (17p6 primer) and XpYp (XpYpE2 primer)
Genomic DNA (500 pg/reaction) for the ciHHV-6-associated telomere (DR1R primer)

dependent variables

Telomere lengths

control variables

Known size markers (GeneRuler 1 kb and GeneRuler High Range DNA ladder, Fermentas)

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!