Characterization of T Cell Activation and Maturation

The proportion of T cells expressing activation (HLA-DR and CD38) and maturation (CD27 and CD45R0) markers was determined in fresh, anti-coagulated whole blood as previously described [25 (link)]. Briefly, fresh blood samples were incubated for 30 min using the following fluorochrome-labelled monoclonal antibodies (mABs): CD3-Pacific Blue (BD), CD4 Per-CP Cy5.5 (eBioscience), CD8 V500 or CD8 Amcyan, CD27 APC-H7, CD45RO APC, HLA-DR FITC and CD38 PE (all from BD). Acquisition was performed on a FACS CANTO II (BD). Compensation was conducted with antibody capture beads (BD) stained separately with the individual antibodies used in the test samples. Flow cytometry data was analysed using FlowJo (version 9.5.3; Tree Star Inc.).

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Nicoli F., Chachage M., Clowes P., Bauer A., Kowour D., Ensoli B., Cafaro A., Maboko L., Hoelscher M., Gavioli R., Saathoff E, & Geldmacher C. (2016). Association between different anti-Tat antibody isotypes and HIV disease progression: data from an African cohort. BMC Infectious Diseases, 16, 344.

Publication 2016

CD3-Pacific Blue CD4 Per-CP Cy5.5 CD27 APC-H7 CD45RO APC HLA-DR FITC CD38 PE FACS CANTO II antibody capture beads

Antibodies Antibody Blood Cd45ro Cy5 5 Fitc Flow cytometry Fluorochrome Hla dr Monoclonal antibodies T cells Tree

Corresponding Organization :

Other organizations : Ludwig-Maximilians-Universität München, National Institute for Medical Research, Istituto Superiore di Sanità, University of Ferrara

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