Monocyte Immune Modulation Assay

CD14⁺ monocytes were selected from PBMCs using CD14-microbeads (Miltenyi) and cells (5 ×10⁵) were stimulated with IFN-γ (100 U/ml, Biolegend) and concanavalin (ConA, Sigma-Aldrich, at 5 μg/ml) as previously described (50 (link), 51 (link)) in the presence or not of etanercept (10 μg/ml), isotype control, IL-17 [10 ng/ml, PreproTech, (52 (link))], IL-10 [50 ng/ml, R&D systems, (26 (link))] or TNF-α (50 ng/ml, Euromedex) and incubated for 9 days.

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Mezouar S., Diarra I., Roudier J., Desnues B, & Mege J.L. (2019). Tumor Necrosis Factor-Alpha Antagonist Interferes With the Formation of Granulomatous Multinucleated Giant Cells: New Insights Into Mycobacterium tuberculosis Infection. Frontiers in Immunology, 10, 1947.

Publication 2019

CD14-microbeads IFN-γ concanavalin (ConA IL-17 IL-10

Cells Cona Etanercept Ifn γ Il 10 Il 17 Isotype Microbeads Monocytes Tnf α

Corresponding Organization : Méditerranée Infection Foundation

Other organizations : Institut des Sciences du Mouvement Etienne-Jules Marey

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Variable analysis

independent variables

Etanercept (10 μg/ml)
Isotype control
IL-17 (10 ng/ml)
IL-10 (50 ng/ml)
TNF-α (50 ng/ml)

dependent variables

Not explicitly mentioned

control variables

CD14+ monocytes selected from PBMCs using CD14-microbeads
Cell count (5 × 10^5 cells)
IFN-γ (100 U/ml)
Concanavalin A (ConA, 5 μg/ml)
Incubation time (9 days)

positive controls

IFN-γ (100 U/ml)
Concanavalin A (ConA, 5 μg/ml)

negative controls

Isotype control

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