miRNA-mRNA Regulatory Associations

Spearman’s correlation analysis was used to identify correlations between miRNAs and mRNA targets by ranking the expression values of miRNAs and mRNAs within datasets and calculating the correlation based on these ranks in order to discern potential regulatory associations^{30 (link)}. One proportion test was performed for each of the miRNAs as we previously described. The predicted targets that were significant by one proportion test were used for IPA. The one proportion test was calculated by significantly inversely correlated targets subtracted from total significantly correlated targets over the total significantly correlated mRNA targets (https://www.medcalc.org/calc/test_one_proportion.php). Statistical analyses are performed using Prism GraphPad version 6 (GraphPad Software, Inc., La Jolla, CA). The alpha value < 0.05 was considered statistically significant.