Quantitative Proteomics and Cell Viability Assays

In-gel fluorescence was recorded using an ETTAN
Dige Imager (GE Healthcare). Chemiluminescence was recorded using
a LAS-3000 Imaging System (Fujifilm). Absorbance in 96-well plates
was measured using a SpectraMax M2/M2e Microplate Reader from Molecular
devices. Culture media and reagents were obtained from Sigma-Aldrich,
Gibco (Life technologies) and A&E Scientific (PAA). For quantitative
proteomics (SILAC), R10K8 and R0K0 DMEM media were purchased from
Dundee cell products, and the cell dissociation buffer (enzyme free,
PBS-based) was obtained from Gibco (Life Technologies). Dialyzed FBS
was obtained from Sigma-Aldrich. All buffers were filtered using a
0.2 μM filter to prevent any contamination. MTS assay was performed
as previously described.^{3 (link)}