Extracellular Recording of Rat Retinal Ganglion Cells

Retinal tissue was obtained from adult (8 weeks old) male Long-Evans rat (Rattus norvegicus) and continuously perfused with Ames Solution (Sigma-Aldrich) and maintained at 32 °C. Ganglion cell spikes were recorded extracellularly from a multi-electrode array with 252 electrodes spaced 60 μm apart (custom fabrication by Innovative Micro Technologies, Santa Barbara, CA). Experiments were performed in accordance with institutional animal care standards. The microelectrode covered a total retinal area of ∼ 1 mm². For the rat this corresponds to 16-17 degrees of visual angle [25 (link)]. The spike sorting was performed with an in-house method based on [22 (link)].

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Botella-Soler V., Deny S., Martius G., Marre O, & Tkačik G. (2018). Nonlinear decoding of a complex movie from the mammalian retina. PLoS Computational Biology, 14(5), e1006057.

Publication 2018

Ames Solution

Adult Ames Animal Cell Ganglion Long evans rat Male Microelectrode Rattus norvegicus Retinal Tissue

Corresponding Organization :

Other organizations : Institute of Science and Technology Austria, Institut de la Vision, Max Planck Institute for Intelligent Systems

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Ganglion cell spikes recorded extracellularly from a multi-electrode array

control variables

Retinal tissue obtained from adult (8 weeks old) male Long-Evans rat (Rattus norvegicus)
Retinal tissue continuously perfused with Ames Solution (Sigma-Aldrich) and maintained at 32 °C
Experiments performed in accordance with institutional animal care standards

controls

Positive control: None mentioned
Negative control: None mentioned

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