Phytocannabinoid and Terpene Analysis by HPLC and GC-MS

HPLC (High performance liquid chromatography, 1260 Infinity II, Agilent) equipped with a Raptor ARC-18 for LC-UV column (150 mm × 4.6 mm ID, pore size 2.7 µm) was used to analyze phytocannabinoids content in each fraction as described previously (Peeri et al., 2021 (link); Shalev et al., 2022 (link)). For chemical analysis of terpenes, 1 μL of each sample was analyzed by a gas chromatography-mass spectrometer (GC8860-MS5977B Agilent) equipped with 30 m, 0.25 mm ID, 5% cross-linked phenylmethyl siloxane capillary column (HP-5MS) with 0.25-μm film thickness, was used as described in (Peeri et al., 2021 (link)).

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Shalev N., Kendall M., Kumar N., Tiwari S., Anil S.M., Hauschner H., Swamy S.G., Doron-Faingenboim A., Belausov E., Kendall B.E, & Koltai H. (2024). Integrated transcriptome and cell phenotype analysis suggest involvement of PARP1 cleavage, Hippo/Wnt, TGF-β and MAPK signaling pathways in ovarian cancer cells response to cannabis and PARP1 inhibitor treatment. Frontiers in Genetics, 15, 1333964.

Publication 2024

1260 Infinity II GC8860-MS5977B

Corresponding Organization : Agricultural Research Organization

Other organizations : Bar-Ilan University

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Variable analysis

independent variables

Type of column used for HPLC analysis (Raptor ARC-18 for LC-UV column)
Column dimensions (150 mm × 4.6 mm ID, pore size 2.7 µm)
Type of instrument used for HPLC analysis (1260 Infinity II, Agilent)
Type of instrument used for GC-MS analysis (GC8860-MS5977B Agilent)
Type of capillary column used for GC-MS analysis (30 m, 0.25 mm ID, 5% cross-linked phenylmethyl siloxane capillary column (HP-5MS) with 0.25-μm film thickness)

dependent variables

Phytocannabinoids content in each fraction
Terpenes content in each sample

control variables

Sample preparation and extraction methods as described in the referenced studies (Peeri et al., 2021 and Shalev et al., 2022)

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