Generating 3'-UTR Reporter Vectors

3′-UTR reporter vectors were generated by inserting 3′-UTR sequences into the 3′-UTR of the luciferase gene in the psiCHECK-2 dual luciferase reporter vector (Promega) (Suzuki et al. 2015 (link), 2018 (link)). Mutations were introduced by a PCR-based approach. Primer sequences are given in Supplemental Table S2.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kelly T.J., Suzuki H.I., Zamudio J.R., Suzuki M, & Sharp P.A. (2019). Sequestration of microRNA-mediated target repression by the Ago2-associated RNA-binding protein FAM120A. RNA, 25(10), 1291-1297.

Publication 2019

psiCHECK-2 dual luciferase reporter vector

3 utr Gene Luciferase Mutations Primer Promega Vector

Corresponding Organization :

Other organizations : Massachusetts Institute of Technology, University of California, Los Angeles

Top 5 similar protocols

Variable analysis

independent variables

3'-UTR sequences inserted into the 3'-UTR of the luciferase gene in the psiCHECK-2 dual luciferase reporter vector
Mutations introduced by a PCR-based approach

dependent variables

Luciferase reporter activity

control variables

PsiCHECK-2 dual luciferase reporter vector

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!