Integrin α6 Antibody Characterization

J8H, a mouse mAb, recognizes an extracellular epitope of α6 integrin and was a generous gift from Dr. Arnoud Sonnenberg (27 (link)). The integrin α6 rat mAb J1B5 was generated by Dr. Caroline Damsky (29 (link)). AA6NT a rabbit pAb, was generated against a recombinant fragment of the N-terminal integrin α6 β-barrel domain and was used for immunohistochemistry (IHC) analysis of archival material. In contrast, AA6A is a rabbit pAb, recognizing the intracellular C-terminal domain of α6 integrin was previously characterized by us and used for western blot analysis (21 (link)). Donkey anti-mouse Alexa conjugated antibodies and anti-Rabbit HRP antibodies were obtained from Invitrogen (Carlsbad, CA). Human, single chain, activated, urokinase was obtained from Millipore (Temecula, CA). Growth Factor Reduced Matrigel was from BD Biosciences (Bedford, MA).

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Ports M.O., Nagle R.B., Pond G.D, & Cress A.E. (2009). Extracellular Engagement of α6 Integrin Inhibited uPA Mediated Cleavage and Delayed Human Prostate Bone Metastasis. Cancer research, 69(12), 5007-5014.

Publication 2009

Anti antibodies Donkey Epitope Growth factor Human Immunohistochemistry Integrin Intracellular Matrigel Mouse Rabbit Urokinase Western blot analysis

Corresponding Organization :

Other organizations : University Radiology, University of Arizona

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Variable analysis

independent variables

J8H, a mouse mAb, recognizing an extracellular epitope of α6 integrin
J1B5, an integrin α6 rat mAb generated by Dr. Caroline Damsky
AA6NT, a rabbit pAb generated against a recombinant fragment of the N-terminal integrin α6 β-barrel domain
AA6A, a rabbit pAb recognizing the intracellular C-terminal domain of α6 integrin

dependent variables

Immunohistochemistry (IHC) analysis of archival material
Western blot analysis

control variables

Donkey anti-mouse Alexa conjugated antibodies
Anti-Rabbit HRP antibodies
Human, single chain, activated, urokinase
Growth Factor Reduced Matrigel

positive controls

None specified

negative controls

None specified

Annotations

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