Resazurin reduction was used to assess the short-term effects of drug treatment. Sterile 0.15 mg/ml resazurin stock was prepared in PBS. Cells were seeded in 96-well plates in RPMI-1640 at least 24 hours before the experiment. The medium was replaced with fresh RPMI-1640 mixed with resazurin stock 1:6 and supplemented with a drug or vehicle control. Cells were incubated for 90 min, and resorufin fluorescence was measured (560 nm excitation and 590 nm emission). The resazurin reduction assay was not used as a proxy for cell count in the present study.
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