RNA from lung tissues and cells was acquired using the RNA Extraction Kit (N065, China). Next, to quantify the mRNA expression, cDNA synthesis was conducted in line with instructions of the cDNA first chain synthesis kit (N118, China) and miRNA cDNA first-strand synthesis kit (KR211, China), respectively. Then, cDNA was amplified and quantitatively analyzed by the FastFire qPCR PreMix (FP207-02, Tiangen) in a C1000 Touch PCR system (E1138, Bio-Rad, USA). GAPDH and U6 were employed for normalization controls. The results were normalized by the 2-ΔΔCt method [20 (link)]. The primers were listed in Table 1 .
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