Quantification of COX-2 and BDNF Gene Expression

Gene expressions of COX-2 and BDNF were measured as previously reported [48 (link)]. Briefly, after extraction through the TRI Reagent, total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific). Gene expression was determined by quantitative real-time PCR using TaqMan probes obtained from Thermo Fischer Scientific. β-actin was used as the housekeeping gene. The analysis of data was conducted with the Sequence Detection System (SDS) software version 2.3 (Thermo Fischer Scientific). A detailed description of the experimental protocol is reported in a previous paper of ours [51 (link)].

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Chiavaroli A., Balaha M., Acquaviva A., Ferrante C., Cataldi A., Menghini L., Rapino M., Orlando G., Brunetti L., Leone S., Recinella L, & di Giacomo V. (2021). Phenolic Characterization and Neuroprotective Properties of Grape Pomace Extracts. Molecules, 26(20), 6216.

Publication 2021

High Capacity cDNA Reverse Transcription Kit TaqMan probes Sequence Detection System (SDS) software version 2.3

Actin Cdna Cox 2 Gene expression Housekeeping gene Quantitative real time pcr Reverse transcription

Corresponding Organization : University of Chieti-Pescara

Other organizations : Kafrelsheikh University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Gene expressions of COX-2
Gene expressions of BDNF

control variables

β-actin (used as the housekeeping gene)

controls

Positive control: None mentioned
Negative control: None mentioned

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